题 目：Bioassessment and DNA Barcoding: Current and Future Practices in Biomonitoring and Stressor Detection
报告人：Dr. Eric Stein, Principal Scientist（Southern California Coastal Water Research Project）
主持人：杨凯 教授、车越 教授
时 间：2015年10月23日（周五）上午10:00
地 点：闵行校区 生科辅楼城市生态实验室一楼119会议室
报告人简介：Dr. Stein is a Principal Scientist at the Southern California Coastal Water Research Project (SCCWRP), where he is the head of the Biology Department. Dr. Stein oversees a variety of projects related to in-stream and coastal water quality, hydromodification, development of biological indices, and assessment of wetlands and other aquatic resources. His research focuses on effects of human activities on the condition of aquatic ecosystems, and on developing tools to better assess and manage those effects. Dr. Stein serves on numerous technical workgroups, committees, and scientific advisory panels at the Federal, State, and local levels related to water quality and wetland assessment and management. Dr. Stein has research experience in a variety of disciplines related to environmental assessment, including multimedia distribution of environmental contaminants, stormwater monitoring and assessment, wetland functional assessment, intestinal nutrient transport kinetics, and physiological ecology. Before joining SCCWRP, Dr. Stein worked as both a private consultant and for the Regulatory Branch of the Los Angeles District Corps of Engineers on issues related to wetlands and water quality management and regulation. Dr. Stein’s experience includes wetland delineation, design of mitigation and restoration projects, development of monitoring programs, establishment of mitigation banks, and landscape-scale assessment.
报告简介：Traditional measures of water or soil chemistry provide a limited view of environmental health through the presence of pollutants or contaminants of concern at a specific point in time and space. In contrast biological measures provide an integrated view of the condition or health of a specific environment through direct measure of the resident organisms that are exposed to contaminants of concern through multiple pathways (e.g. ambient contact, ingestion). In aquatic environments, such as streams, wetlands, and coastal waterbodies, primary producer communities (e.g. algae) and primary consumer communities (e.g. benthic invertebrates) are often used as bioassessment indicators. Changes in the composition and structure of these resident communities not only provide a more robust measure of overall health, but are useful for “rapid” screening of large numbers of sites to provide assessments of ambient (or watershed health) and to prioritize sites for management intervention.
Over the past 20 years, numerous indices have been developed worldwide that provide simple measures of health (or condition) based on algal and benthic invertebrate community composition. Although these tools have been broadly successful, they have some limitations. Molecular tools, such as DNA barcoding and metabarcoding provide promise for fulfilling the needs for “next generation assessments.” The use of standardized DNA sequence markers – DNA barcodes – has become a common, standard practice in many areas of biodiversity assessment. Customized, public databases of DNA barcodes and other marker gene sequences (e.g., BOLD, GenBank) contain representative DNA barcodes for hundreds of thousands of animal, plant, fungal, and microbial taxa. Comparison of DNA barcodes recovered from unidentified specimens can be used to provide species-level identification for a wide range of organisms. In addition to DNA barcode regions, molecular methods have also been employed to investigate functional gene regions of both prokaryotes and eukaryotes. The combination of increased taxonomic resolution and direct measure of function holds promise for improving the diagnostic power of bioassessment.
In this talk, Dr. Stein will explore current approaches to bioassessment as well as next-generation molecular methods in their ability to assess condition and diagnose stress. He will also discuss some of the science needed to advance these tools to broadscale application for environmental monitoring and assessment.
欢迎老师和同学们参加！
